Intermediate

Part:BBa_K539624:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Taiwan   (2011-09-27)

a RBS (B0030)+ilvD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1742
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1528
    Illegal BsaI site found at 1785


Design Notes

Built in strong expressing RBS (Part:BBa_B0030) and ilvD(Part:BBa_K539712). The sequence of ilvD had been mutated to delet the function of RE site at the origin in order to apply to the iGEM part.

Source

The sequence of ilvD comes from Escherichia coli str. K-12 substr. MG1655

References